Cellular localization of the wild-type FANCC protein and the L554P mutant. (A) The HeLa cell line expresses low levels of endogenous FANCC protein that is undetectable by anti-FANCC immunofluorescence. HeLa cells were infected with retroviral supernatants, pMMP-FANCC (B) or pMMP-FANCC-L554P (C). Pools of infected cells were stained with anti FANCC and the DNA-specific dye, DAPI (4′,6-diamidino-2-phenylindole) (bottom images) and analyzed by immunofluorescence, as previously described.4