SLC and ELC but not RANTES induced chemotaxis of mature DC. (A and B) Migration of human monocyte-derived DC matured with MCM (▪) or TNF- (▨) compared to untreated immature cells (□), in response to the indicated concentrations (in ng/mL) of (A) SLC or (B) ELC. The percent migration (±SE) is based on the number of cells added to the top chamber. (C) Migration of MCM-treated mature DC or untreated (immature) DC in response to the indicated concentrations (in ng/mL) of RANTES. Chemotactic index is the ratio of the number of cells in the bottom chamber in the test case to the number of cells in the absence of the chemokine. (D) Preincubation with 100 ng/mL pertussis toxin (PT) inhibited SLC-induced migration of MCM-treated mature DC. These results are representative of at least three independent experiments.