Calcium mobilization in mature DC induced by SLC and ELC. DC (A) without or (B through E) with MCM-treatment were loaded with Fluo-3AM. The chemokines were added at the times indicated by the arrows and the calcium responses analyzed by flow cytometry. (A through C) Calcium responses to 500 ng/mL of SLC, ELC, or RANTES. (D and E) Dose-dependent calcium responses induced by 500, 150, 50, 15, 5, and 1.5 ng/mL of (D) SLC or (E) ELC. (F and G) Mature DC were loaded with Fluo-3AM and calcium mobilization was analyzed by flow cytometry. Concentration of the chemokines added were: (F) 100 ng/mL SLC, 500 ng/mL ELC, (G) 500 ng/mL ELC, 100 ng/mL SLC. These results are representative of two or more independent experiments.