Chemokine receptor expression profile in DC. Total RNA was prepared from DC after 8 days of culture, either untreated (−) or treated with MCM (+) from days 5-8. First-strand cDNA were synthesized and used in semi-quantitative PCR analysis. PCR products from 30 cycles of amplification were visualized on an ethidium-bromide–stained 2% agarose gel. Molecular-weight markers (in kb) are shown to the left of the gels. The annealing temperatures for all PCR reactions were 55°C except for CCR5, CCR6, CCR9, CXCR3, CXCR5, CX₃CR1, which were at 60°C. These results are representative of seven or more experiments using three independent pairs of cDNA.