Kinetics of Sn⁺ macrophage accumulation in livers of tumor-bearing DBA/2 mice under ADI. Frozen tissue sections were single-stained for total Kupffer cells (F4/80⁺) and Sn⁺ Kupffer cells (A) or double-stained for F4/80⁺/B7.1⁺ and for Sn⁺/B7.1⁺ (B) with corresponding MoAbs. Six lobules per liver were analyzed under the microscope. (A) The number of Sn⁺ (▪) and F4/80⁺ (▴) Kupffer cells in ESb-MP tumor-bearing untreated (d −1, 5 Gy; d0, no immune cells) mice and the number of Sn⁺ (◊) and F4/80⁺(○) Kupffer cells in ADI-treated (d −1, 5 Gy; d0, 2 × 10⁷ B10.D2 immune spleen cells) animals are shown. (B) The number of Sn⁺ (◊), Sn⁺/B7.1⁺ (⧫), F4/80⁺(○), and F4/80⁺/B7.1⁺ (•) in ADI-treated mice are shown. Data present the means and standard deviations from two experiments with 2 to 3 animals per time point. Data points without SD represent mean values for which the SD was smaller than 1.