Flow cytometric analyses of Ly-6A/E and FcγRII/III expression by pooled primary colony cells. (A and H) Isotype control. (B) Expression of Ly-6A/E and FcγRII/III by pooled primary colony cells and sorting windows for Ly-6A/E⁻ and Ly-6A/E⁺ cells. (C) Sorted Ly-6A/E⁻ cells. (D) Sorted Ly-6A/E ⁺ cells. (E) Expression of Ly-6A/E and FcγRII/III by pooled primary colony cells and sorting windows for FcγRII/ III^−/low and FcγRII/III^high cells. (F) Sorted FcγRII/III^−/low cells. (G) Sorted FcγRII/III^high cells. (I) Expression of Ly-6A/E and FcγRII/III by pooled primary colony cells and sorting windows for Ly-6A/E⁺ FcγRII/III^−/low cells and the rest of the cells. (J and K) Sorted cells using the windows presented in (I).