Capping/clustering of CD34 or CD43 on normal CD34⁺ cells. Normal CD34⁺ BM cells were stimulated with anti-CD34 (4A1) for 0 minutes (a through c) and 30 minutes (d through f), or with anti-CD43 (MEM59) for 0 minutes (g through i) and 30 minutes (j through l). After fixation, cells were double-stained with anti-CD34 (green channel: a and d) and phalloidin (red channel: b and e) or with anti-CD43 (green channel: g and j) and phalloidin (red channel: h and k), and analyzed by confocal immunofluorescence microscopy. The right-hand panels show superimposed images of green and red channels. Bar, 5 μm.