Identification of the VEGF-induced factor binding to OL6. Radioactively labeled OL6 was incubated with nuclear extracts from unstimulated cells (lane 1) and cells treated with VEGF (lanes 2 to 8) in the absence or presence of unlabeled oligonucleotides and specific antibodies. The Sp1 (complexes I and II), VEGF-induced (complex IV), and nonspecific (ns) protein/DNA complexes are indicated. VEGF-stimulated extracts were analyzed by competition with a 100-fold molar excess of unlabeled oligonucleotides OL6 (lane 3), OL1 (lane 4), or AP-1 (see Materials and Methods, lane 5). Supershift experiments were performed using 3 μL of anti–EGR-1 (lane 6), anti–AP-1 (lane 7), or anti–AP-2 (lane 8) antibodies. Shown is one experiment that is representative of three experiments with similar results.