A point mutation 5′ to the EGR-1 consensus site abrogates EGR-1 binding. Sequences of the wild-type (OL6) and mutant (mOL6) are shown at the top. The consensus EGR-1 site is underlined and the G → A substitution is indicated by a bold letter. Nuclear extracts from unstimulated (−) and VEGF-stimulated (+) cells were incubated with radiolabeled OL6 or mOL6. The position of the EGR-1 complex is indicated. Shown is one experiment that is representative of three experiments with similar results.