Differential binding of nuclear proteins to -1234C/T, -1185A/G, and -1051G/A allele sequences. (A) EMSAs performed with nuclear extracts from unstimulated BAECs. Arrow, -1234C/T-specific factor; arrowhead, -1051A allele-specific factor; free, free (unbound) probe. Allele sequences for -1234C/T and -1051G/A exhibited differential DNA-protein complex formation, while the -1185A/G sequences did not appear to bind factors present in BAEC nuclear extracts. (B) EMSAs performed with recombinant human NFκB (p50). Arrow, oligo/rhNFκB p50 complex formation; arrowhead, supershift with anti-NFκB (p50) antibody (Ab). Markedly enhanced binding of rNFκB p50 occurred to the -1234T allelic probe; this complex was supershifted by antibodies directed against NFκB p50. A NFκB consensus oligonucleotide was included in this EMSA as a positive control.