Flow cytometric analysis of endothelial adhesion molecule after IκB- overexpression. (A) Untreated HUVEC were stained with MoAbs to ICAM-1 (stippled line), VCAM-1 (dashed line), E-selectin (dotted line), and isotype control (solid line). (B through D) Cells stimulated with TNF- (100 U/mL) for 4 hours were stained with MoAbs (solid line) to ICAM-1 (B), VCAM-1 (C), E-selectin (D), or isotype control (B through D, dotted lines). Cells infected with rAd.IκB- and stimulated with TNF- (100 U/mL for 4 hours) were stained with MoAbs (stippled line) to ICAM-1 (B), VCAM-1 (C), E-selectin (D), or isotype control (B through D, dashed lines). Shown is a representative experiment.