Fig. 3.
HIV-TCM increases VEGF-A secretion in HUT 78 cells. HUT 78 cells (1 × 106) were incubated in medium containing 25% of TCM or HIV-TCM. Cell culture supernatants were harvested after 1, 3, and 5 days of incubation and analyzed (in duplicate) for the presence of VEGF-A by ELISA. The data were subtracted of the VEGF-A levels present in TCM and HIV-TCM. In comparison to TCM-treated cells, VEGF-A concentrations were 1.8- to 2.0-fold higher in HUT 78 cells incubated with HIV-TCM after 1 day (□), 3 days (▩), and 5 days (▪) of incubation, respectively.
