SH-TCM increases VEGF-A expression in HUT 78 cells. (A) Northern blot analysis of VEGF-A mRNA expression in HUT 78 cells. Cells were incubated with buffer (control) or SH-TCM. Total RNA was isolated after 5 days of incubation, subjected to electrophoresis (20 μg per lane), and blotted onto a nylon membrane. Hybridization was performed with a radioactively labeled human VEGF-A–specific probe at 42°C for 16 hours. VEGF-A mRNA expression in HUT 78 cells increased by threefold after incubation with SH-TCM as compared with buffer. (B) Ethidium bromide staining of blotted RNA indicated that equal amounts of RNA were used in each lane. (C) Densitometric evaluation of band intensities from the Northern blot shown in (A).