In situ hybridization analysis of GATA-2 mRNA expression in early mouse embryos. GATA-2 mRNA expression was observed in the lateral mesoderm adjacent to primitive streak of 7.5 dpc embryo (A and B) and AGM region in 9.5 dpc embryo (C and D) with antisense probe (A and C), but not with sense probe (B and D). Arrowhead in panel A indicates the lateral mesoderm, and in panel C an arrow shows the splanchnopleura. Panels E and F are coronal sections of 9.5 and 10.5 dpc embryos analyzed with the antisense probe. Abbreviations are a, dorsal aorta; m, mesonephros; n, neural tube. Original magnifications are ×50 (E) and ×25 (F).