Hematopoietic cell-specific expression of GATA-2 mRNA in the liver and spleen of embryos and neonates. The expression of GATA-2 mRNA in the fetal liver of 12.5 dpc embryo (A) as well as in the liver of 3-day (B) or 7-day (C) postnatal pups was analyzed by in situ hybridization analysis on tissue sections. In 5-day old pups, GATA-2 mRNA-positive cells were observed in the red pulp but not in the white pulp (R and W, respectively) of the spleen (D and E). The results with antisense (A-E) and sense (F) probes are shown. Arrowheads in panels A, B, D, and E indicate positive cells. Original magnifications are ×64 (A-C, E) and ×20 (D and F).