DNase I hypersensitive sites in the upstream region of the IS and IG exons of GATA-2 gene. The top panel shows the position of DNase I hypersensitive sites in the 5′ region of the locus. Long arrows represent major hypersensitive sites and short arrows represent minor sites. Probe positions are also shown (a and b). Genomic DNA treated with DNase I were digested byKpnI/SacI (A),XhoI/SacI (B), orNotI/SalI (C), and hybridized with probe a (A and B) or probe b (C). Two major hypersensitive sites were found in the upstream region of the IS exon and two minor sites were also found at 1.8 and 1.5 kbp upstream from the SacI site. The SacI site is 0.4 kbp downstream from the transcription start site of IS exon. One major hypersensitive site exists in 3.0 kbp downstream from the NotI site in the IS exon (C).