Hematopoietic and neural tissue-specific transcription in the 7.0ISGFP transgenic mouse. Whole-mount analysis of GFP expression in 7.0ISGFP transgenic mouse embryos (line 398). GFP-positive cells were identified in the 8.5 dpc yolk sac (A) and 9.0 (B), 9.5 (C), 10.5 (D), and 11.5 (E) dpc embryos. Green fluorescence-positive cells are observed in para-aortic splanchnopleura (red arrows), liver rudiment (white arrows), and vitelline vessels (arrowheads). In panel E , the GFP brightly positive cells are in the ventral side of the neural tube. Panel F shows a dorsal view of the embryo in panel E. These embryos were also analyzed after dissection. GFP-positive cells are observed in para-aortic splanchnopleura in 9.5 dpc embryo (G) and in or near the dorsal aortic wall of 10.5 dpc embryo (H). GFP-positive cells are also observed in 11.5 dpc fetal liver (I). Upper limb buds were eliminated to show the staining in the trunks.