American Society of Hematology

Fig. 9.

$Fig. 9. GFP-expression recapitulates endogenous IS promoter activity. GFP-positive and -negative cell fractions were sorted by FACS from the liver of 11.5 dpc embryo of 7.0ISGFP transgenic mouse. Endogenous expression of the IS exon of mouse GATA-2 gene was analyzed using RT-PCR. The primer pairs amplifying the elongation factor (EF) 1 mRNA was used as a control.38$

GFP-expression recapitulates endogenous IS promoter activity. GFP-positive and -negative cell fractions were sorted by FACS from the liver of 11.5 dpc embryo of 7.0ISGFP transgenic mouse. Endogenous expression of the IS exon of mouse GATA-2 gene was analyzed using RT-PCR. The primer pairs amplifying the elongation factor (EF) 1 mRNA was used as a control.38

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