Permissivity of mature DC to HIV. (A) Expression of HIV coreceptors: Culture day 8 sorted CD1a⁺ DC were cultured for 48 hours under standard conditions, with or without CD40LT, and labeled with FITC-CCR5 or PE-CXCR4 MoAbs; solid histograms: labeling with an irrelevant MoAb; open histograms: staining by the relevant MoAb; representative data of one of four experiments. (B) Nested PCR detection of viral DNA in HIV-infected mature DC: DC that had been cultured for 48 hours with GM-CSF/TNF-/IL-4 and CD40LT were exposed to HIV-1_Ba-L or HIV-1_LAI; cell lysates were prepared 48 hours PI. Comparative endpoint dilution analysis was performed as in Fig 2; results of one experiment of five.