Effect on virus production of coculturing HIV-1_Ba-L–infected immature DC with autologous CD4⁺ TL. (A) Effect of activated CD4⁺ TL (n = 9): 72 hours PI with HIV-1_Ba-L, DC were cocultured or not (□) with resting (◊) or CD3 MoAb-activated (○) autologous CD4⁺ TL (T4L) in the presence of AZT; virus production is expressed as mean ± SD Log10 HIV RNA copy numbers; differences were not statistically significant (paired Student’s t-test) at any time point when comparing DC only versus DC + resting CD4⁺ TL; differences were significant when comparing DC + CD3-activated CD4⁺ TL versus DC + resting CD4⁺ TL (24 hours: P = .02; 48 hours: P= .02; 96 hours: P = .04), or versus DC only (24 hours:P = .05; 48 hours: P = .04; 96 hours: P = .02). (B) Virus transmission from DC to CD3-activated CD4⁺ TL (n = 4): 72 hours PI with HIV-1_Ba-L, DC were cocultured (○/•) or not (□/▪) with CD3 MoAb-activated T4L in the presence (open symbols) or absence (black symbols) of AZT; differences between AZT⁺ and AZT⁻ conditions were not significant (paired Student’st-test) as regards DC cultured alone; in cocultures, differences were significant at 48 hours (P = .04) and 96 hours (P = .001).