HIV infection of DC derived from CD1a⁺CD14⁻ or CD1a⁻CD14⁺ precursors. (A) Expression of HIV coreceptors by immature and mature DC of either population: CD1a⁺CD14⁻ and CD1a⁻CD14⁺ DC precursors sorted on day 5 were cultured for 48 hours with SCF/FL/GM-CSF/TNF-/IL-4 with or without CD40LT, and labeled with FITC-CCR5 or PE-CXCR4 MoAbs; open and solid histograms are as in Fig 4A; representative composite results from two experiments of five. (B) Immature DC derived from either sorted CD1a⁺ CD14⁻ (□) or CD1a⁻CD14⁺ (○) precursors were cultured for 48 hours under the standard condition before exposure to HIV-1_Ba-L, the production of which is expressed as geometric mean ± SD ng p24/10⁵ viable cells; differences were not statistically significant at any time point (n = 5).