Fig. 5.
Three-color immunofluorescence analysis of TEL/AML1 leukemia bone marrow cells shows cell surface expression of ΨL in the absence of μ chain. Leukemic blasts coexpressing CD34 and CD19 were gated (R1) and analyzed for the other markers. Cells were stained with PerCP-labeled anti-CD34, FITC-labeled anti-CD19, and PE-labeled anti-VpreB or PerCP-labeled anti-CD34, PE-labeled anti-CD19, and either FITC-labeled anti-CD10, anti-CD40, or anti-CD24 MoAbs. For μ and κ/λ intracellular staining, cells were first labeled with PerCP-labeled anti-CD34 and PE-labeled anti-CD19 and then permeabilized and treated with FITC-conjugated rabbit antihuman IgM F(ab′)2 or FITC-conjugated rabbit antihuman κ F(ab′)2 plus antihuman λ F(ab′)2.
