Effect of deglycosylation on components of the proB-cell complex. Cell surface labeling and immunoprecipitation conditions are as described in Fig 6. The anti-VpreB 4G7 immunoprecipitates from the JEA2 proB and the anti-μ immunoprecipitates from the NALM6 preB-cell lines were incubated with or without PNGase F, submitted to a gradient SDS-PAGE (5% to 15%) under reducing conditions, and transferred onto immobilon P membrane. Cell surface biotinylated proteins were detected by streptavidin-peroxidase and the membrane was incubated with the anti-VpreB 4G7 MoAb and shown by a peroxidase-conjugated goat antimouse IgG.