0.8% to 1.7% of Lin⁻/34⁺/DR^dim cells are myeloid-lymphoid initiating cells. Progeny from single Lin⁻/34⁺/DR^dim cells cultured on AFT024 in expansion medium with Flt3-L, SCF, and IL-7 were replated after 4 weeks in 8 secondary plates. Four secondary plates were maintained in expansion medium with Flt3-L, SCF, and IL-7 to enumerate LTC-IC. The remaining 4 secondary plates were maintained in lymphoid differentiation medium to detect NK-IC. Lin⁻/34⁺/DR^dim cells that generated ≥1 LTC-IC (black circles) and ≥1 NK-IC (gray circles) were identified as ML-IC.