Fig. 5.
Effects of antioxidant inhibitors on the ability of RBCs to inhibit neutrophil apoptosis. RBCs were pretreated with the catalase inhibitor aminotriazole (AMT), the glutathione metabolism inhibitors chlorodonitrobenzene (CDNB) and mercaptosuccinate (MS), a combination of AMT, CDNB, and MS, or the O2− transport inhibitor DIDS. Excess reagents were removed by washing RBCs three times in PBS. Then, RBCs were mixed with neutrophils at a neutrophil/RBC ratio of 1:10. After 48 hours of culture, neutrophil apoptosis was evaluated as described in Fig 1. Data represents mean ± SEM of five experiments. **P < .01 versus untreated RBCs.
