SDS-PAGE analysis (A) of purified ACA cofactors of patients no. 4, 5, and 6 recovered after Sephacryl S-300 chromatography (B). The three effluents from this column were monitored for protein content and cofactor activity as described in Materials and Methods. Fractions were pooled as indicated by the horizontal bars. The elution profile of plasma proteins is depicted at top of (B). Electrophoresis was performed in 7.5% nonreducing and reducing polyacrylamide gels and then stained with Coomassie blue. Arrows indicate molecular weight markers.