Kinetics of BCL3 upregulation by IL-9. BW5147 cells were cultured in the absence or in the presence of 100 U/mL IL-9 for the indicated periods of time. Total RNA was extracted and RT-PCR amplification was performed with oligonucleotides specific forBCL3 or β-actin. A Southern blot of the PCR products was performed and filters were hybridized with the BCL3 orβ-actin cDNA probes.