IL-9 increases NF-κB DNA binding. (A) EMSA analysis of IL-9–stimulated BW5147 cells. Cells were cultured in the absence or in the presence of 100 U/mL IL-9. Nuclear extracts were prepared after various stimulation times and a mobility shift assay was performed using a 32P-labeled palindromic κB site. An Sp1 DNA binding oligonucleotide was used as a control for the loading of similar protein quantities in each lane. (B) Western blot analysis of BCL3 induction by IL-9. The left panel shows BCL3 expression in BW5147 cells transfected with a control vector (−) or the BCL3 cDNA cloned into the pEF-BOS plasmid (BCL3) as a positive control. In the right panel, cells were cultured as in (A). Total extracts were prepared and analyzed by Western blot with anti-BCL3 antibodies as described in Materials and Methods.
