Efficiency of transduction of recombinant adenoviruses into WEHI-3B cells. (Panel I) β-gal staining of cells transduced with virus at moi of (a) 4, (b) 40, (c) 400, and (d) 4,000. (Panel II) Flow cytometric analysis of fluorescein-stained cells transduced with virus at moi of (A) 0, (B) 4, (C) 40, (D) 400, and (E) 4,000 and exposed to 20 mmol/L fluorescein di-β-D-galactopyranoside (FDG) at 37°C for 1 hour. The rate of transduction of cells at each moi was (A) 0.38%, (B) 0.55%, (C) 1.20%, (D) 3.74%, and (E) 29.69%.