Flow cytometric analysis and cytotoxicity assay of spleen cells from mice receiving BMMNCs from animals injected with cytokine-expressing WEHI-3B cells. (A) Schematic diagrams of biological analysis of mice transfused with modified BMMNCs. (B) Expression of CD80 (B7-1) as well as CD86 (B7-2) in the spleen cells. Control (isotype IgG), mouse injected with modified BMMNCs by WEHI-3B–lacZ, mouse injected with modified-BMMNCs by WEHI-3B–GM-CSF plus WEHI-3B–IL-4, and mouse injected with modified BMMNCs by WEHI-3B–GM-CSF plus WEHI-3B–TNF. (C) Cytotoxic activity by spleen cells of mouse injected with modified-BMMNCs by WEHI-3B–GM-CSF plus WEHI-3B–IL-4. (D) Cytotoxic activity by spleen cells of mouse injected with modified-BMMNCs by WEHI-3B–GM-CSF plus WEHI-3B–TNF.