Western blot analysis of total Max and of Mad1:Max complexes during induced differentiation of U-937-myc-6 cells. U-937-myc-6 cells were induced with TPA, IFN-γ, or TPA + IFN-γ for the times indicated. (Upper panel) Max was coimmunoprecipitated from low stringency lysates using C19 Mad1 antibodies followed by Western blot analysis of Max by using C17 Max antibodies (Lower panel). Max was immunoprecipitated from aliquots of the same lysates with C17 Max antibodies followed by immunoblot analysis using the same antibodies.