Fig. 5.
Fig. 5. The 5′-UTR is required for expression of theFlk-1 gene in the yolk sac vasculature. Transgenic mouse embryos that carry a Flk-1 promoter and 5′-UTR (−640 bp/+299 bp)/enhancer (+1677 bp/+3947 bp) reporter gene construct show a uniform vascular expression in the yolk sac vasculature (A and B). In contrast, the yolk sac of Flk-1/LacZ knock-in embryos that lack part of the 5′-UTR (+137 bp to +299 bp) shows expression only in large collecting vessels that connect with the embryo, but not in the smaller vessels (C). Transgenic yolk sacs that carry a Flk-1 promoter (−640 bp/+137 bp) / enhancer (+1677 bp/+3947 bp) reporter gene construct show a diminished and incomplete vascular LacZ expression (D) when compared with the construct containing the complete 5′-UTR. (A and B) Bar, 500 μm.

The 5′-UTR is required for expression of theFlk-1 gene in the yolk sac vasculature. Transgenic mouse embryos that carry a Flk-1 promoter and 5′-UTR (−640 bp/+299 bp)/enhancer (+1677 bp/+3947 bp) reporter gene construct show a uniform vascular expression in the yolk sac vasculature (A and B). In contrast, the yolk sac of Flk-1/LacZ knock-in embryos that lack part of the 5′-UTR (+137 bp to +299 bp) shows expression only in large collecting vessels that connect with the embryo, but not in the smaller vessels (C). Transgenic yolk sacs that carry a Flk-1 promoter (−640 bp/+137 bp) / enhancer (+1677 bp/+3947 bp) reporter gene construct show a diminished and incomplete vascular LacZ expression (D) when compared with the construct containing the complete 5′-UTR. (A and B) Bar, 500 μm.

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