Subcellular localization of Nramp2 in enterocytes. Tissue sections from the proximal duodenum (I1; Fig 2) from mice maintained on a low iron diet were analyzed for Nramp2 expression. Immunochemistry (A and B) or immunofluorescence (C and D) were performed on sections using the anti-Nramp2 NT antibody. Crypts of Lieberkuhn (A and C) and villi (B and C) are shown. Nramp2 staining was observed in the brush border (arrow) and intracellularly (arrowhead), whereas goblet cells (white arrow) and lamina propria remain negative. (Original magnification × 1,000.)