Fig. 3.
Detection of HHV-8 DNA in purified cell populations from PBMC of homosexual men with HIV infection. Aliquots of cell lysates (105 cells) were subjected to PCR with primer set 3.PCR products were analyzed by Southern blot hybridization with a32P-labeled oligonucleotide probe internal to the amplified sequences. Shown are the results with B cells (B), monocytes (M), or T cells (T) from 2 AIDS-KS and 2 NKS-AIDS patients. Cell populations were purified as described and purification was monitored by FACS.All samples were positive for β-globin amplification (data not shown). An HIV+ patient was positive in B cells (data not shown). The NKS-AIDS and the HIV+ patients were positive for anti–HHV-8 Ab by IFA.
