Fig. 7.
![Fig. 7. Effects of various agents on [3H]Sph metabolism in HUVECs. HUVECs were incubated with [3H]Sph for 1 minute and then challenged without (C) or with 100 U/mL of IL-1β (IL-1), 1 U/mL of thrombin (Thr), or 1 μmol/L TPA for 20 minutes in the presence of a 1% FCS medium. Lipids were extracted from cells or media and analyzed for [3H]Sph metabolism. Locations of standard lipids are indicated on the left.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/93/12/10.1182_blood.v93.12.4293/4/blod41226007w.jpeg?Expires=1735830419&Signature=qFXMm-JHOK~G1dem4wfuPv38DXTaI2Qq4kmbEqPgpJndCt7pXyVgc0RFSR5tgzizIiYGgo-lHuGgq~s3ayYNCQi5QKQGv3CkHmcZsUd0vjsv8CQQd2GYR~Mx~0BjzXHGdN~3U6WJ1K8yitYeGVUWKUkQ1cqYTBKG0cUxXA6KQsHUrZt4F1OLsMO4THK3HIMBZ80YmyHmOOOLJ0Y5bN4KK6fpo0TmhkFmiXQ7OpTor1K2Et3NrFawZuFoNKjdy-5fcEkU1OrFrZKavc6ZiHD9pkqmSiFyN2DPU8psGf4wjyrlfVIuet7aYkyPCQg~YLXv-Io2W5xJ-oipXECy-DR4KQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effects of various agents on [3H]Sph metabolism in HUVECs. HUVECs were incubated with [3H]Sph for 1 minute and then challenged without (C) or with 100 U/mL of IL-1β (IL-1), 1 U/mL of thrombin (Thr), or 1 μmol/L TPA for 20 minutes in the presence of a 1% FCS medium. Lipids were extracted from cells or media and analyzed for [3H]Sph metabolism. Locations of standard lipids are indicated on the left.
