American Society of Hematology

Fig. 5.

$Fig. 5. C1 and C2 trigger translocation of mitochondrial Cyt.C in HL60 and M14 cells. (A) HL60 (30 × 106 cells) were treated with 100 μg/mL of C1 or C2 for 12 hours and cytosolic fractions were subjected to SDS-PAGE electrophoresis, transferred to PVDF membrane, and subjected to Western blot analysis for Cyt.C as described in Materials and Methods. Anti–β-actin antibody was used to assess equal loading of samples. (B) M14 cells (1 × 103) were grown on cover slips and treated with 100 μg/mL of C1 or C2 for 12 hours, and Cyt.C localization was determined by confocal microscopy using anti-Cyt.C as described in Materials and Methods.$

C1 and C2 trigger translocation of mitochondrial Cyt.C in HL60 and M14 cells. (A) HL60 (30 × 10⁶ cells) were treated with 100 μg/mL of C1 or C2 for 12 hours and cytosolic fractions were subjected to SDS-PAGE electrophoresis, transferred to PVDF membrane, and subjected to Western blot analysis for Cyt.C as described in Materials and Methods. Anti–β-actin antibody was used to assess equal loading of samples. (B) M14 cells (1 × 10³) were grown on cover slips and treated with 100 μg/mL of C1 or C2 for 12 hours, and Cyt.C localization was determined by confocal microscopy using anti-Cyt.C as described in Materials and Methods.

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