Effect of the PKC inhibitor, GF109203X, on neutrophil apoptosis. (A) Neutrophils were incubated in the absence (control) or presence of GF109203X (1 μmol/L), G-CSF (50 ng/mL), or GM-CSF (10 ng/mL) and apoptosis quantified by annexin V binding/flow cytometry at 24 and 48 hours. Mean ± standard error (SE) of 11, 10, and 8 separate experiments for GF109203X, G-CSF, and GM-CSF, respectively. (B) Concentration response curve for effects of GF109203X on neutrophil survival. Cell death was measured at 24 hours by annexin V binding and flow cytometry. Mean ± SE of three separate experiments.