PKC isoforms expressed in neutrophils and HL-60 cells. Cell lysates from unstimulated freshly isolated neutrophils (PMN) or HL60 cells (20 μg of total protein were loaded per lane) were immunoblotted with various antibodies specific for PKC isoforms. Isoforms that were probed for, but not detected, in either cell type were PKCγ, ɛ, θ. Neutrophil/HL60 expression of the ERK2 MAPkinase is shown for comparison. Lysates from one of two separate experiments analyzed are shown.