Incubation of neutrophils ex vivo leads to the generation of a C-terminal proteolytic fragment of PKCδ. Lysates prepared from neutrophils aged for the indicated times. Cells (20 μg of total protein loaded per lane) were analyzed by immunoblotting using an antibody that recognizes the C-terminal end of PKCδ (A) or antibodies to the C-terminal portion of PKCs  and βII (B).