Fig. 1.
Expression of PAI-1 mRNAs in UT-7 cell lines. Total cellular RNAs were extracted from UT-7 cell lines (UT-7/OR, UT-7/EPO, UT-7/GM, and UT-7/TPO) and expressions of PAI-1 mRNA were evaluated by RT-PCR (see Materials and Methods). The RT-PCR products were resolved by agarose gel electrophoresis, and the bands were transferred to a membrane and hybridized to 32P-labeled PAI-1 or β-actin cDNA probes.
