American Society of Hematology

Fig. 5.

$Fig. 5. Proliferative reactive plasma cells are mainly located in the CD138− plasmablastic fraction. The labeling index was determined by BrdU incorporation, as described in Fig 4. The labeling index was determined in the unseparated population and the CD138+ and CD138− populations. CD138+ and CD138− cell isolation was performed as described in Materials and Methods (RP10). Acquisition was performed either in R1 gated cells (A and B) or R2 gated cells (C and D). (A and C) λ-PE versus BrdU-FITC; (B and D) λ-PE versus control IgG1-FITC.$

Proliferative reactive plasma cells are mainly located in the CD138⁻ plasmablastic fraction. The labeling index was determined by BrdU incorporation, as described in Fig 4. The labeling index was determined in the unseparated population and the CD138⁺ and CD138⁻ populations. CD138⁺ and CD138⁻ cell isolation was performed as described in Materials and Methods (RP10). Acquisition was performed either in R1 gated cells (A and B) or R2 gated cells (C and D). (A and C) λ-PE versus BrdU-FITC; (B and D) λ-PE versus control IgG1-FITC.

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