TCR CDR3 size distribution (spectratype) analysis of overall peripheral TCRBV repertoires in CD4⁺ and CD8⁺ T-cell subsets after sorting for MHC class II expression. (A) Results of the TCR CDR3 size distribution analysis in patient 1 before allo-BMT, ie, MHC class II–negative fractions in CD4⁺ and CD8⁺ T-cell subsets. (B) Patient 1 one year after allo-BMT, T-cell subsets divided in MHC class II–expressing (MHC II+) or lacking (MHC II−) T-cell fractions. (C) Donor 1. From left to right, TCRBV families 1-23 are depicted in CD4⁺ and CD8⁺ T-cell subsets.