FACS profiles of embryonic cells. The cells were isolated from the liver of 10.5 dpc embryo or from the rest of the body and stained by immunofluorescence for each of the antibody markers shown as described in Materials and Methods. Three antibodies are the same rat IgG2b isotype. No staining above the autofluorescence background was observed with an irrelevant antibody of the same isotype. The unstained cells in the upper two panels were stained with second antibody alone. Numbers on each panel represent the percentage of the total cell population falling within the quadrant indicated. A total of 104 cells was counted in each experiment. The overall pattern is representative of two independent cell isolations from a litter of pooled embryos at 10.5 dpc.
