Western blotting analysis of MSH2 protein in leukemic cells. Thirty micrograms of protein was loaded per lane and blotted with either anti-MSH2 antibody or anti-actin antibody as a control. In the upper panel, the expression of MSH2 protein was shown in the patients from left to right: 1 (JH), 2 (MD), 3 (WA), 4 (CA), 5 (DC), 6 (SR), 7 (PN), 8 (DG), 9 (MC), and 10 (PS). In the lower panel, no MSH2 protein was shown in the patients from left to right A (EH), B (EW), C (KP), D (JG), E (MJ), F (MP), G (GMT), H (MT), I (NS), J (RG), K (SG), L (AH),M(AS), and N (BG). The expression of actin as a control was shown in all of these patients.