(A) HT-2.EPOR cells express EPOR mRNA. Ten micrograms of cytoplasmic RNA from HT-2.EPOR.3 and HT-2 cells was separated by denaturing agarose gel electrophoresis, transferred to Zeta Probe membranes and probed with a ³²P-labeled 1.2-kb DNA fragment corresponding to the EPOR extracellular domain. (B and C) The EPOR protects HT-2 cells from cell death and apoptosis. HT-2 and HT-2.EPOR.3 cells were incubated for 40 hours with no cytokine (None), 20 U/mL or indicated doses of EPO (Epo), or 10 nmol/L IL-2 (IL-2). Cells were costained with PI and Annexin-GFP and analyzed by flow cytometry.