Chemotactic response of RSMC to pro-u-PA, C-su-PAR, and peptide 1. Chemotaxis assay was performed as described in methods using modified Boyden chambers. (A) Comparison of the chemotactic effects of pro-u-PA (1 nmol/L) and fMLP (10−7 mol/L) on RSMC. (B) Effects of increasing doses of pro-u-PA on RSMC migration. (C) Effects of increasing concentrations of C-su-PAR on RSMC migration. (D) Chemotactic activity of synthetic peptides on RSMC migration. Peptide 1 (AVTYSRSRYLEC), which corresponds to amino acids 84-95 of human u-PAR, and a scrambled version of peptide 1 (TLVEYYSRASCR) were tested as chemoattractant. Cells migrated towards increasing doses of peptide 1 or its scrambled version, as indicated. (E) Effect of the LRP antagonist RAP on RSMC migration and its effect on pro-u-PA stimulation. (F) Influence of either wild-type BPT or mutated BPT on pro-u-PA–induced or bFGF-induced chemotactic response of RSMC. Toxins were present in both chambers of the Boyden apparatus. Random cell migration of unstimulated cells is considered to be 100% migration. Results are the mean ± SD (n = 3).
