Effects of chymotrypsin-cleaved su-PAR and peptide 1 on actin cytoskeleton on RSMC. Cells were incubated in the absence of chemoattractant (A) or with 10 pmol/L C-su-PAR for 5 (B) or 30 minutes (C) or with 1 pmol/L peptide 1 for 30 minutes. Effects of BPT on the pro-u-PA–induced actin cytoskeleton reorganization on RSMC. Cells were untreated or pretreated with 50 ng/mL pertussis toxin or with 50 ng/mL of mutated pertussis toxin for 6 hours; the cells were then incubated with or without 1 nmol/L pro-u-PA in the absence or in the presence of toxin. (A) Untreated and unstimulated cells. (E) Unstimulated cells treated with 50 ng/mL pertussis toxin. (F) Unstimulated cells treated with 50 ng/mL mutated pertussis toxin. (G) Untreated cells stimulated for 30 minutes with 1 nmol/L pro-u-PA. (H) Pertussis toxin (50 ng/mL) -treated cells stimulated for 30 minutes with 1 nmol/L pro-u-PA. (I) Mutated pertussis toxin (50 ng/mL)- treated cells stimulated for 30 minutes with 1 nmol/L pro-u-PA. Actin filaments were visualized using fluorescein-conjugated phalloidin.