Effect of antibodies anti-u-PAR and anti-VNR on RSMC chemotaxis in response to pro-u-PA and fMLP. Cells were detached from support and pretreated for 1 hour at 4°C in serum-free medium without or with antibodies against u-PAR (10 μg/mL) or with antibodies anti-VNR (LM 609) (0.5 μg/mL) or with unspecific isotype-matched control antibodies (10 μg/mL). The cells were then subjected to chemotaxis assay and migrated towards medium alone (□), 10 nmol/L pro-u-PA (▪), or 10−7 mol/L fMLP (▨). Antibodies at the same concentrations as in the pretreatment were added in both chambers of the Boyden apparatus and were present during the whole assay. Random cell migration of RSMC towards medium alone without any chemoattractants or antibodies was considered to be 100% migration. Results are the mean mean ± SD (n = 3).
