A hematopoietic stem cell line and megakaryocytic cell lines do not express β-catenin and cadherin. Western blot analysis of total cell lysates (100 μg protein per lane) from CTS, CMS, CMK, and Dami cells untreated or PMA (100 nmol/L)-treated for 48 hours was performed using anti–β-catenin antibody (1 μg/mL; A) and anticadherin (1 μg/mL) antibody (B). COS-7 cells were used as a positive control. The positions of migration of β-catenin and cadherin are shown.