Identification of clonally related T-cell subsets in the skin (A), peripheral blood (B), and bone marrow (C) from a representative MM patient (UPN 14). (Upper panel) CDR3 size distribution at the level of BV23-BC transcripts. (X-axis) Sizes in amino acids of the CDR3 regions. (Y-axis) Fluorescence intensities, reflecting the number of clones using each CDR3 size combination. Three recurrent predominant peaks (7 aa, 9 aa, and 10 aa) are identified in each sample. (Lower panel) CDR3 size distribution at the level of BV23-BJ transcripts. The usage of individual BJ segments in the 7 aa, 9 aa, and 10 aa peaks from the skin (A1-A3), peripheral blood (B1-B3), and bone marrow (C1-C3) samples are shown.